Application of High Performance Thin Layer Chromatography for Qualitative Analysis of Isoflavones in Medicinal Plants
نویسندگان
چکیده
The retention behavior of commonly occurring compounds with estrogenic activity: daidzein, formononetin, genistein, prunetin, biochanin A and coumestrol on silica and silica modified with cyjanopropyl, aminopropyl, diol and octadecyl groups was investigated. Mixtures of methanol or acetonitrile with water and eluents such as chloroform, heptane and toluene with more polar modifiers: ethyl acetate, acetone, diisopropyl ether in various concentrations were used as mobile phases. The best result of separation was obtained on RP-18s plates with use diisopropyl ether/toluene/formic acid (40/60/0.5 v/v/v) as eluent. All investigated compounds were well separated which enabled their densitometric analysis. The proposed chromatographic system was used to qualitative analysis of investigated compounds in plant material.
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